University of Leipzig (1972-1976)
Diploma in Biochemistry (1976), University of Leipzig
Title (translated): Investigations on the interaction of yeast alcohol dehydrogenase and its hydrophobic substrates
(Prof. H. Aurich)
Doctorate in Biochemistry (1985), University of Leipzig
Title (translated): Development and application of a radioimmunoassay for 2,4-dichlorophenoxyacetic acid
(Prof. P. Nuhn)
University lecturing qualification (habilitation) in Applied Biochemistry (1992), University Halle-Wittenberg
Title (translated): The immunological determination of 2,4-D in body fluids of exposed persons - a biological exposure test for the calculation of individual health risk
(Prof. P. Nuhn)
Postgraduation (1982-1986) Academy of physicians education, Berlin
Topic (translated): Medical toxicology/Toxicological chemistry
Visiting Scientist (1989), Laboratory of Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
Topic: Immunoaffinity chromatography
(Dr. R. Stephany)
Research scientist at the Department of Environmental Toxicology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1976-1989)
Head of the Department of Experimental Toxicology and Epidemiology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1989-1990)
Since 1991: Research Group at the Technical University of Munich, Institute of Hydrochemistry and Chemical Balneology (Prof. Dr. R. Niessner)
2002: Position of Academic Director
2005: Appointment apl. Professor
Journals:
- Ecotoxicology and Environmental Safety
- Chromatographia
- International Journal of Environmental Research and Public Health
Kupstat, A., Knopp, D., Niessner, R., Kumke M.U. "Novel intramolecular energy transfer probe for the detection of Benzo[a]pyrene metabolites in a homogeneous competitive fluorescence immunoassay" J. Phys. Chem. B 114 (2010) 1666-1673.
Tang, D., Sauceda, J.C., Lin, Z., Ott, S., Basova, E., Goryacheva, I., Biselli, S., Lin, J., Niessner, R., Knopp, D. "Magnetic nanogold microsperes-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food" Biosensors & Bioelectronics 25 (2009) 514-518.
Tang, D., Zhong, Z., Niessner, R., Knopp, D. "Double-codified gold nanolabels with enhanced electrochemical immunoassay for rapid detection of aflatoxin B1 in food using multifunctional magnetic beads as probes" The Analyst 134 (2009) 1509-1728.
Knopp, D., Tang, D., Niessner, R. "Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles" Analytica Chimica Acta 647 (2009) 14-30.
Rieger, M., Cervino, C., Sauceda, J. C., Niessner, R., Knopp, D. "Efficient Hybridoma Screening Technique Using Capture Antibody Based Microarrays" Anal. Chem. 81 (2009) 2373-2377.
Tang, D., Niessner, R., Knopp, D. "Flow-injection electrochemical immunosensor for the detection of human IgG based on glucose oxidase-derived biomimetic interface" Biosensors and Bioelectronics 24 (2009) 2125 - 2130.
Beloglazova, N.V., Goryacheva, I.Y., Mikhirev, D.A., De Saeger S., Niessner, R. and Knopp, D. "New immunochemically-based field test for monitoring benzo[a]pyrene in aqueous samples" Analytical Sciences 24 (2008) S. 1613-1617.
Liu, J.J., Lin, J.M. and Knopp, D. "Using a circular groove surrounded inlet to generate monodisperse droplets inside a microfluidic chip in a gravity-driven manner" Journal of Micromechanics and Microengineering 18 (2008) articel number 095014.
Cervino, C., Sauceda, J.C., Niessner, R. and Knopp, D. "Mycotoxin analysis by automated flow-through immunoassay with chemiluminescence readout" Luminescence 23 (2008) 206 -207.
Sun, Z., Niessner, R. and Knopp, D. "Selective trace analysis of dichlofenac in surface and wastewater samples using solid phase extraction with a new molecularly imprinted polymer" Analytica Chimica Acta 629 (2008) 73 - 81.
Nießner, R., Broekaert, J.A., Einax, J., Emons, H., Haisch, C., Huber, C., Jakubowski, N., Knopp, D., Popp, J., Scheller, F.W., and Schuhmann, W. "Analytische Chemie 2006/2007", Nachrichten aus der Chemie 4 (2008) 418 - 427.
Cervino, C., Asam, S., Knopp, D., Rychlik, M. and Niessner, R. "Use of isotope-labeled aflatoxins for LC-MS/MS stable isotope dilution analysis of foods" Journal of Agricultural and Food Chemistry 56 (2008) 1873 - 1879.
Goryacheva, I.Y. Beloglazova, N.V., Eremin, S. A., Mikhirev, D.A., Niessner, R. and Knopp, D. "Gel-based immunoassay for non-instumental detection of pyrene in water samples" Talanta 75 (2008) 517 - 522.
Stolper, P., Fabel, S., Weller, M.G., Knopp, D. and Niessner, R. "Whole-cell luminescence-based flow-through biodetector for toxicity testing" Analytical and Bioanalytical Chemistry 390 (2008) 1181 - 1187.
Cervino, C., Weber, E., Knopp, D. and Niessner, R. "Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies" Journal of Immunological Methods 329 (2008) 184 - 193.
Miller, J., Niessner, R., and Knopp, D., "Enzymes and Immunoassays" In: Ullmann's Biotechnology and Biochemical Engineering, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Vol. 2, 2007, S. 586 - 612.
Lai, J., Yang, M., Niessner, R., and Knopp, D., "Molecularly imprinted microspheres and nanospheres for di(2-ethylhexyl)phthalate prepared by precipitation polymerisation" Analytical and Bioanalytical Chemistry 389 (2007) 2, 405 - 412.
Han, D., Yang, X., Knopp, D., Niessner, R., Wu., M., Pu., C. and Deng, A., "Development of a highly sensitive and specific enzyme-linked immunosorbent assay for detection of Sudan I in food samples" Journal of Agricultural and Food Chemistry 55 (2007) 6424 - 6430.
Goryacheva, I.Y., Eremin, S.A., Shutaleva ,E.A., Suchanek, M., Niessner, R., Knopp, D., "Development of a fluorescence polarization immunoassay for polycyclic aromatic hydrocarbons" Analytical Letters 40 (2007) 3267 - 3272.
Belmont, A.-S., Jäger, S., Knopp, D., Niessner, R., Gauglitz, G., Haupt, K., "Molecularly imprinted polymer films for reflectometric interference spectroscopic sensors" Biosensors and Bioelectronics 22 (2007) 3267 - 3272.
Knopp, D., Deng, A., Letzel, M., Himmelbach, M., Zhu, Q.-Z., Peröbner, I., Kudlak, B., Frey, S., Sengl, M., Buchberger, W., Taggart, M., Hutchinson, C., Cunningham, A., Pain, D., Cuthbert, R., Raab, A., Meharg, A., Swan, G., Jhala, Y., Prakash, V., Rahmani, A., Quervedo, M., Niessner, R., “Immunological determination of the pharmaceutical Diclofenac in environmental and biological samples.” In: National Environmental Management of Agrochemicals: Risk Assessment, Monitoring and Remedial Action" ACS Symposium Series, ACS Washington, 2007, Vol. 966, 203-226.
Cervino, C., Knopp, D., Weller, M.G., Niessner, R., “Novel aflatoxin derivates and protein conjugates” Molecules 12 (2007) 641-653.
Knopp, D. “Detection of trace metal ions in environmental matrices by immunological methods – a review” Ecological Chemistry and Engineering 13 (2006) 383-397.
Tsagkogeorgas, F., Ochsenkühn-Petropoulou, M., Niessner, R. and Knopp, D. „Encapsulation of biomolecules for bioanalytical purposes – preparation of antibody-doped nanometer-sized silica particles by reverse micelle and sol-gel processing“ Analytica Chimica Acta 573-574 (2006) 133-137.
Knopp, D. „Immunoassay development for environmental analysis – Trends.“ Analytical and Bioanalytical Chemistry 385 (2006) 425-427.
Degelmann, P., Egger, S., Jürling, H., Müller, J., Niessner, R., Knopp, D. „Determination of sulfonylurea herbicides in water and food samples using sol-gel glass based immunoaffinity extraction and LC-UV/DAD or LC-MS/MS” Journal of Agricultural and Food Chemistry 4 (2006) 2003–2011.
Matschulat, D., Haider, F., Niessner, R. and Knopp, D. ”Immunization with soot from a non-combustion process provokes formation of antibodies against polycyclic aromatic hydrocarbons” Journal of Immunological Methods 310 (2006) 159-170.
Eremin, S. A., Knopp, D., Niessner, R., Hong, J. Y., Park, S.-J. and Choi, M. J. “High throuput determination of BTEX by one-step fluorescence polarization immunoassay” Environmental Chemistry 2 (2005) 227-234.
Matschulat, D., Deng, A., Niessner, R. and Knopp, D. „Development of a highly sensitive monoclonal antibody based ELISA for benzo[a]pyrene in potable water” The Analyst 130 (2005) 1078-1086.
Knopp, D. “CH. XIII.: Analysis of 2,4-Dichlorophenoxyacetic Acid in body fluids of exposed subjects using radioimmunoassay” In: Pesticide Protocols (Eds.: J.L. Martinez Vidal, A.G. Frenich) Humana Press, Totowa, New Jersey, 2005, pp 119-131.
(c) 2010 Institute of
Hydrochemistry,
Technische Universität München, Marchioninistr. 17, D-81377 München
phone: +49 89 2180-78231, fax: +49 89 2180-78255,
reinhard.niessner@ch.tum.de