University of Leipzig (1972-1976)
Diploma in Biochemistry (1976), University of Leipzig
Title (translated): Investigations on the interaction of yeast alcohol dehydrogenase and its hydrophobic substrates
(Prof. H. Aurich)
Doctorate in Biochemistry (1985), University of Leipzig
Title (translated): Development and application of a radioimmunoassay for 2,4-dichlorophenoxyacetic acid
(Prof. P. Nuhn)
University lecturing qualification (habilitation) in Applied Biochemistry (1992), University Halle-Wittenberg
Title (translated): The immunological determination of 2,4-D in body fluids of exposed persons - a biological exposure test for the calculation of individual health risk
(Prof. P. Nuhn)
Postgraduation (1982-1986) Academy of physicians education, Berlin
Topic (translated): Medical toxicology/Toxicological chemistry
Visiting Scientist (1989), Laboratory of Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
Topic: Immunoaffinity chromatography
(Dr. R. Stephany)
Research scientist at the Department of Environmental Toxicology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1976-1989)
Head of the Department of Experimental Toxicology and Epidemiology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1989-1990)
Since 1991: Research Group at the Technical University of Munich, Institute of Hydrochemistry and Chemical Balneology (Prof. Dr. R. Niessner)
2002: Position of Academic Director
2005: Appointment apl. Professor
Journals:
- Ecotoxicology and Environmental Safety
- Chromatographia
- International Journal of Environmental Research and Public Health
Tang, J., Tang, D., Niessner, R., Knopp, R., Chen, G. "Hierarchical dendritic gold microstructure-based aptasensor for ultrasensitive electrochemical detection of thrombin using functionalized mesoporous silica nanospheres as signal tags" Analytica Chimica Acta 720 (2012) 1-8.
Karsunke, X.Y.Z., Wang, H., Weber, E., McLean, M.D., Niessner, R., Hall, J.C., Knopp, D. "Development of single-chain variable fragment (scFv) antibodies against hapten benzo[a]pyrene: a binding study" Anal. Bioanal. Chem. 402 (2012) 499-507.
Saini, M, Taggert, M. A., Knopp, D., Upreti, S., Swarup, D., Das, A., Gupta, P. K., Niessner, R., Prakash, V., Mateo, R., Cuthbert, R. J. "Detecting diclofenac in livestock carcasses in India with an ELISA: A tool to prevent widespread vulture poisoning" Environ. Pollut. 160 (2012) 11-16.
Beloglazova, N. V., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Comparison of different labels for non-instrumental gel-based immunoassay" Microchimica Acta 175 (2011) 361-367.
Karsunke, X. Y. Z., Pschenitza, M., Rieger, M., Weber, E., Niessner, R., Knopp, D. "Screening and characterization of new monoclonal anti-benzo[a]pyrene antibodies using automated flow-through microarray technology" J. Immunol. Methods 371 (2011) 81-90.
Beloglazova, N. V., Goryacheva, I. Y., de Saeger, S., Scippo, M. L., Niessner, R., Knopp, D. "New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical test column test" Talanta 85 (2011) 151-156.
Tang, J., Tang, D., Niessner, R., Chen, G., Knopp, D. "Magneto-controlled graphene immunosensing platform for simultaneous multiplexed electrochemical immunoassay using distinguishable signal tags" Anal. Chem. (2011) 5407-5414.
Tang, J., Tang, D., Niessner, R., Knopp, D. "A novel strategy for ultra-sensitive electrochemical immunoassay of biomarkers by coupling multifunctional iridium oxide (IrOx) nanospheres with catalytic recycling of self-produced reactants" Anal. Bioanal. Chem. (2011) 2041-2051.
Sauceda-Friebe, J. C., Karsunke, X. Y. Z., Vazac, S., Biselli, S., Niessner, R., Knopp, D. "Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection" Analytica Chimica Acta 689 (2011) 234-242.
Tang, D.,Yu, Y., Niessner, R., Miro, M., Knopp, D. "Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles" Analyst 135 (2010) 2661-2667.
Maiolini, E., Knopp, D., Niessner, R., Eremin, S., Bolelli, L., Ferri, E. N., Girotti, S. "Chemiluminescence ELISA for BTEX determination in water and soil" Analytical Sciences 26 (2010) 773-777.
Lin, Z., Sauceda-Friebe, J. C., Lin, J.-M., Niessner, R., Knopp, D. "Double-codified nanogold particles based automated flow-through chemiluminescence immunoassay for 2,4-dinitrotoluene" Analytical Methods 2 (2010) 824-830.
Kupstat, A., Knopp, D., Niessner, R., Kumke M.U. "Novel intramolecular energy transfer probe for the detection of Benzo[a]pyrene metabolites in a homogeneous competitive fluorescence immunoassay" J. Phys. Chem. B 114 (2010) 1666-1673.
Tang, D., Sauceda, J.C., Lin, Z., Ott, S., Basova, E., Goryacheva, I., Biselli, S., Lin, J., Niessner, R., Knopp, D. "Magnetic nanogold microsperes-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food" Biosensors & Bioelectronics 25 (2009) 514-518.
Tang, D., Zhong, Z., Niessner, R., Knopp, D. "Double-codified gold nanolabels with enhanced electrochemical immunoassay for rapid detection of aflatoxin B1 in food using multifunctional magnetic beads as probes" The Analyst 134 (2009) 1509-1728.
Knopp, D., Tang, D., Niessner, R. "Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles" Analytica Chimica Acta 647 (2009) 14-30.
Rieger, M., Cervino, C., Sauceda, J. C., Niessner, R., Knopp, D. "Efficient Hybridoma Screening Technique Using Capture Antibody Based Microarrays" Anal. Chem. 81 (2009) 2373-2377.
Tang, D., Niessner, R., Knopp, D. "Flow-injection electrochemical immunosensor for the detection of human IgG based on glucose oxidase-derived biomimetic interface" Biosensors and Bioelectronics 24 (2009) 2125 - 2130.
Beloglazova, N.V., Goryacheva, I.Y., Mikhirev, D.A., De Saeger S., Niessner, R. and Knopp, D. "New immunochemically-based field test for monitoring benzo[a]pyrene in aqueous samples" Analytical Sciences 24 (2008) S. 1613-1617.
Liu, J.J., Lin, J.M. and Knopp, D. "Using a circular groove surrounded inlet to generate monodisperse droplets inside a microfluidic chip in a gravity-driven manner" Journal of Micromechanics and Microengineering 18 (2008) articel number 095014.
Cervino, C., Sauceda, J.C., Niessner, R. and Knopp, D. "Mycotoxin analysis by automated flow-through immunoassay with chemiluminescence readout" Luminescence 23 (2008) 206 -207.
Sun, Z., Niessner, R. and Knopp, D. "Selective trace analysis of dichlofenac in surface and wastewater samples using solid phase extraction with a new molecularly imprinted polymer" Analytica Chimica Acta 629 (2008) 73 - 81.
Nießner, R., Broekaert, J.A., Einax, J., Emons, H., Haisch, C., Huber, C., Jakubowski, N., Knopp, D., Popp, J., Scheller, F.W., and Schuhmann, W. "Analytische Chemie 2006/2007", Nachrichten aus der Chemie 4 (2008) 418 - 427.
Cervino, C., Asam, S., Knopp, D., Rychlik, M. and Niessner, R. "Use of isotope-labeled aflatoxins for LC-MS/MS stable isotope dilution analysis of foods" Journal of Agricultural and Food Chemistry 56 (2008) 1873 - 1879.
Goryacheva, I.Y. Beloglazova, N.V., Eremin, S. A., Mikhirev, D.A., Niessner, R. and Knopp, D. "Gel-based immunoassay for non-instumental detection of pyrene in water samples" Talanta 75 (2008) 517 - 522.
Stolper, P., Fabel, S., Weller, M.G., Knopp, D. and Niessner, R. "Whole-cell luminescence-based flow-through biodetector for toxicity testing" Analytical and Bioanalytical Chemistry 390 (2008) 1181 - 1187.
Cervino, C., Weber, E., Knopp, D. and Niessner, R. "Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies" Journal of Immunological Methods 329 (2008) 184 - 193.
(c) 2011 Institute of
Hydrochemistry,
Technische Universität München, Marchioninistr. 17, D-81377 München
phone: +49 89 2180-78231, fax: +49 89 2180-78255,
reinhard.niessner@ch.tum.de