Prof. Dietmar Knopp

Head of group



Education (degrees, date, universities):

University of Leipzig (1972-1976)

Diploma in Biochemistry (1976), University of Leipzig
Title (translated): Investigations on the interaction of yeast alcohol dehydrogenase and its hydrophobic substrates
(Prof. H. Aurich)

Doctorate in Biochemistry (1985), University of Leipzig
Title (translated): Development and application of a radioimmunoassay for 2,4-dichlorophenoxyacetic acid
(Prof. P. Nuhn)

University lecturing qualification (habilitation) in Applied Biochemistry (1992), University Halle-Wittenberg
Title (translated): The immunological determination of 2,4-D in body fluids of exposed persons - a biological exposure test for the calculation of individual health risk
(Prof. P. Nuhn)

Postgraduation (1982-1986) Academy of physicians education, Berlin
Topic (translated): Medical toxicology/Toxicological chemistry

Visiting Scientist (1989), Laboratory of Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
Topic: Immunoaffinity chromatography
(Dr. R. Stephany)


Research scientist at the Department of Environmental Toxicology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1976-1989)

Head of the Department of Experimental Toxicology and Epidemiology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1989-1990)

Since 1991: Research Group at the Technical University of Munich, Institute of Hydrochemistry and Chemical Balneology (Prof. Dr. R. Niessner)

2002: Position of Academic Director

2005: Appointment apl. Professor


Membership of Editorial Boards:


  - Ecotoxicology and Environmental Safety

  - Chromatographia

  - International Journal of Environmental Research and Public Health

  - Guest Editor for Special Issue "Biochips" of the journal Sensors (ISSN 1424-8220) (2012)

Period 2010 - 2014

Sharma, A. K., Saini, M., Singh, S. D., Prakash, V., Das, A., Dasan, R. B., Pandey, S., Bohara, D., Galligan, T. H., Green, R. E., Knopp, D., Cuthbert, R. J. "Diclofenac is toxic to the steppe eagle Aquila nipalensis: widening the diversity of raptors threatened by NSAID misuse in South Asia" Bird Conserv. Int. (2014) doi: 10.1017/S0959270913000609.

Pschenitza, M., Hackenberg, R., Niessner, R., Knopp, D. "Analysis of benzo[a]pyrene in vegetable oil using molecularly imprinted solid phase extraction (MISPE) coupled with enzyme-linked immunosorbent assay (ELISA)" Sensors 14 (2014) 9720-9737.

Pschenitza, M., Gavrilova, E. S., Tarasov, S. A., Knopp, D., Niessner, R., Epstein, O. I. "Application of heterogeneous immunoassay for the quality control testing of release-active forms of diclofenac" Int. Immunopharmacol. 21 (2014) 225-230.

Eisold, U., Kupstat, A., Klier, D., Primus, P.-A., Pschenitza, M., Niessner, R., Knopp, D., Kumke, M. U. "Probing the physicochemical interactions of 3-hydroxy-benzo[a]pyrene with different monoclonal and recombinant antibodies by use of fluorescence line-narrowing spectroscopy" Anal. Bioanal. Chem. 406 (2014) 3387-3394.

Speranskaya, E. S., Beloglazova, N. V., Lenain, P., De Saeger, S., Wang, Z., Zhang, S., Hens, Z., Knopp, D., Niessner, R., Potapkin, D. V., Goryacheva, I. Yu. "Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay" Biosensors & Bioelectronics 53 (2014) 225-231.

Tang, D., Liu, B., Niessner, R., Li, P., Knopp, D. "Target-induced displacement reaction accompanying cargo release from magnetic mesoporous silica nanocontainers for fluorescence immunoassay" Anal. Chem. 85 (2013), 10589-10596.

Beloglazova, N. V., Shmelin, P. S., Speranskaya, E. S., Lucas, B., Helmbrecht, C., Knopp, D., Niessner, R., De Saeger, S., Goryacheva, I. Yu. "Quantum dot loaded liposomes as new luminescent labels for immunoassay" Anal. Chem. 85 (2013) 7197-7204.

Oswald, S., Karsunke, X.Y.Z., Dietrich, R., Märtlbauer, E., Niessner, R., Knopp, D. "Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals" Anal. Bioanal. Chem. 405 (2013) 6405-6415.

Li, D., Ying, Y., Wu, J., Niessner, R., Knopp, D. "Comparison of monomeric and polymeric horseradish peroxidase as labels in competitive ELISA for small molecule detection" Microchimica Acta 180 (2013) 711-717.

Tang, D., Hou, L., Niessner, R., Xu, M., Gao, Z., Knopp, D. "Multiplexed electrochemical immunoassay of biomarkers using metal sulfide quantum dot nanolabels and trifunctionalized magnetic beads" Biosens. Bioelectron. 46 (2013) 37-43.

Zhang, B., Tang, D., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Anodic-stripping voltametric immunoassay for ultrasensitive detection of low-abundance proteins using quantum dot aggregated hollow microspheres" Chem. Eur. J. 19 (2013) 2496-2503.

Yu, H.-W., Kim, I. S., Niessner, R., Knopp, D. "Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels" Analytica Chimica Acta 750 (2012) 191-198.

Zhang, B., Liu, B., Tang, D., Niessner, R., Chen, G., Knopp, D. "DNA-based hybridization chain reaction for amplified bioelectronic signal and ultrasensitive detection of proteins" Anal. Chem. 84 (2012) 5392-5399.

Wang, Y., Yang, H., Pschenitza, M., Niessner, R., Li, Y., Knopp, D., Deng, A. "Highly sensitive and specific determination of mercury(II) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody" Anal. Bioanal. Chem. 403 (2012) 2519-2528.

Tang, J., Tang, D., Niessner, R., Knopp, R., Chen, G. "Hierarchical dendritic gold microstructure-based aptasensor for ultrasensitive electrochemical detection of thrombin using functionalized mesoporous silica nanospheres as signal tags" Analytica Chimica Acta 720 (2012) 1-8.

Karsunke, X.Y.Z., Wang, H., Weber, E., McLean, M.D., Niessner, R., Hall, J.C., Knopp, D. "Development of single-chain variable fragment (scFv) antibodies against hapten benzo[a]pyrene: a binding study" Anal. Bioanal. Chem. 402 (2012) 499-507.

Saini, M, Taggert, M. A., Knopp, D., Upreti, S., Swarup, D., Das, A., Gupta, P. K., Niessner, R., Prakash, V., Mateo, R., Cuthbert, R. J. "Detecting diclofenac in livestock carcasses in India with an ELISA: A tool to prevent widespread vulture poisoning" Environ. Pollut. 160 (2012) 11-16.

Beloglazova, N. V., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Comparison of different labels for non-instrumental gel-based immunoassay" Microchimica Acta 175 (2011) 361-367.

Karsunke, X. Y. Z., Pschenitza, M., Rieger, M., Weber, E., Niessner, R., Knopp, D. "Screening and characterization of new monoclonal anti-benzo[a]pyrene antibodies using automated flow-through microarray technology" J. Immunol. Methods 371 (2011) 81-90.

Beloglazova, N. V., Goryacheva, I. Y., de Saeger, S., Scippo, M. L., Niessner, R., Knopp, D. "New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical test column test" Talanta 85 (2011) 151-156.

Tang, J., Tang, D., Niessner, R., Chen, G., Knopp, D. "Magneto-controlled graphene immunosensing platform for simultaneous multiplexed electrochemical immunoassay using distinguishable signal tags" Anal. Chem. (2011) 5407-5414.

Tang, J., Tang, D., Niessner, R., Knopp, D. "A novel strategy for ultra-sensitive electrochemical immunoassay of biomarkers by coupling multifunctional iridium oxide (IrOx) nanospheres with catalytic recycling of self-produced reactants" Anal. Bioanal. Chem. (2011) 2041-2051.

Sauceda-Friebe, J. C., Karsunke, X. Y. Z., Vazac, S., Biselli, S., Niessner, R., Knopp, D. "Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection" Analytica Chimica Acta 689 (2011) 234-242.

Tang, D.,Yu, Y., Niessner, R., Miro, M., Knopp, D. "Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles" Analyst 135 (2010) 2661-2667. 

Maiolini, E., Knopp, D., Niessner, R., Eremin, S., Bolelli, L., Ferri, E. N., Girotti, S. "Chemiluminescence ELISA for BTEX determination in water and soil" Analytical Sciences 26 (2010) 773-777.

Lin, Z., Sauceda-Friebe, J. C., Lin, J.-M., Niessner, R., Knopp, D. "Double-codified nanogold particles based automated flow-through chemiluminescence immunoassay for 2,4-dinitrotoluene" Analytical Methods 2 (2010) 824-830.

Kupstat, A., Knopp, D., Niessner, R., Kumke M.U. "Novel intramolecular energy transfer probe for the detection of Benzo[a]pyrene metabolites in a homogeneous competitive fluorescence immunoassay" J. Phys. Chem. B 114 (2010) 1666-1673.

(c) 2014 Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, D-81377 München
phone: +49 89 2180-78231, fax: +49 89 2180-78255, reinhard.niessner(at), Impressum