Prof. Dietmar Knopp

Head of group



Education (degrees, date, universities):

University of Leipzig (1972-1976)

Diploma in Biochemistry (1976), University of Leipzig
Title (translated): Investigations on the interaction of yeast alcohol dehydrogenase and its hydrophobic substrates
(Prof. H. Aurich)

Doctorate in Biochemistry (1985), University of Leipzig
Title (translated): Development and application of a radioimmunoassay for 2,4-dichlorophenoxyacetic acid
(Prof. P. Nuhn)

University lecturing qualification (habilitation) in Applied Biochemistry (1992), University Halle-Wittenberg
Title (translated): The immunological determination of 2,4-D in body fluids of exposed persons - a biological exposure test for the calculation of individual health risk
(Prof. P. Nuhn)

Postgraduation (1982-1986) Academy of physicians education, Berlin
Topic (translated): Medical toxicology/Toxicological chemistry

Visiting Scientist (1989), Laboratory of Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
Topic: Immunoaffinity chromatography
(Dr. R. Stephany)


Research scientist at the Department of Environmental Toxicology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1976-1989)

Head of the Department of Experimental Toxicology and Epidemiology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1989-1990)

Since 1991: Research Group at the Technical University of Munich, Institute of Hydrochemistry and Chemical Balneology (Prof. Dr. R. Niessner)

2002: Position of Academic Director

2005: Appointment apl. Professor


Membership of Editorial Boards:


  - Ecotoxicology and Environmental Safety

  - Chromatographia

  - International Journal of Environmental Research and Public Health

  - Guest Editor for Special Issue "Biochips" of the journal Sensors (ISSN 1424-8220) (2012)

Period 2012 - 2016

Oswald, S., Dietrich, R., Märtlbauer, E., Niessner, R., Knopp, D. "Mircorarray-based immunoassay for parallel quantification of multiple mycotoxins in oats", In: Methods in Molecular Biology, Vol. 1536 "Oat-Methods and Protocols" (ed. S. Gasparis) (2017) pp 143-156.

Ben Haddada, M., Huebner, M., Casale, S., Knopp, D., Niessner, R., Salmain, M., Boujday, S. "Gold nanoparticles assemply on silicon and gold surfaces: Mechanism, stability and efficiency in diclofenac biosensing" Journal of Physical Chemistry C 120 (2016) 29302-29311.

Lin, Y., Zhou, Q., Tang, D., Niessner, R.,  Yang, H., and Knopp, D. "Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins" Anal.Chem. 88 (2016) 7858 - 7866

Tschofen, M., Knopp, D., Hood, E., Stöger, E. "Plant molecular farming: much more than medicines" Annual Review of Analytical Chemistry 9 (2016) 271-294

Hlaváçek, A., Farka, Z., Hübner, M., Hornákova, V., Nemecek, D., Niessner, R., Skládal, P., Knopp, D., Gorris, H.H. "Competitive upconversion-linked immunosorbent assay for the sensitive detection of diclofenac". Anal. Chem. 88 (2016) 6011-6017

Wang, X., Niessner, R., Tang, D., Knopp, D. "Nanoparticle-based immunosensors and immunoassays for aflatoxins". Anal. Chim. Acta 912 (2016), 10-23.

Neumann, A.-C, Wang, X., Niessner, R., Knopp, D. "Determination of microcystin-LR in surface water by a magnetic-bead-based colorimetric immunoassay using antibody-conjugated gold nanoparticles".  Anal. Methods 8 (2016) 57-63.

Huebner, M., Weber, E., Niessner, R., Boujday, S., Knopp, D., "Rapid analysis of diclofenac in freshwater and wastewater by a monoclonal antibody-based highly sensitive ELISA". Anal. Bioanal. Chem. 407 (2015) 8873-8882.

Knopp, D. "Future Food Forensics". The Analytical Scientist 32 (2015) 17-18.

Wang, X., Pauli, J., Niessner, R., Resch-Genger, U., Knopp, D. "Gold nanoparticle-catalyzed uranine reduction for signal amplification in fluorescent assays". Analyst 140 (2015) 7305-7312.

Gao, Z., Tang, D., Tang, D., Niessner, R., Knopp, D. "Target-induced nanocatalyst deactivation facilitated by core@shell nanostructures for signal-amplified headspace-colorimetric assay of dissolved hydrogen sulfide".  Analytical Chemistry 87 (2015) 10153-10160.

Lin, Y., Zhou, Q., Lin, Y., Tang, D., Niessner, R., Knopp, D. "Enzymatic hydrolysate-induced displacement reaction with multifunctional silica beads doped with oxidoreductase and mediator for ultrasensitive electrochemical immunoassay".  Analytical Chemistry 87 (2015) 8531-8540.

Lux, G., Langer, A., Pschenitza, M., Karsunke, X., Strasser, R., Niessner, R., Knopp, D., Rant, U. "Detection of the carcinogenic water pollutant Benzo[a]pyrene with electro-switchable biosurface". Analytical Chemistry 87 (2015) 4538-4545.

Wang, X., Niessner, R., Knopp, D. "Controlled growth of immunogold for amplified optical detection of aflatoxin B1". Analyst 140 (2015) 1453-1458.

Huebner, M., Haddada, M. B., Méthivier, C., Niessner, R., Knopp, D., Boujday, S. "Layer-by-layer generation of PEG-based regenerable immunosensing surfaces for small-sized analytes". Biosens. Bioelectron. 67 (2015) 334-341.

Mercier, D., Haddada, M. B., Huebner, M., Knopp, D., Niessner, R., Salmain, M., Proust, A., Boujday, S. "Polyoxometalate nanostructured gold surfaces for sensitive biosensing of benzo[a]pyrene". Sensors & Actuators B 209 (2015) 770-774.

Tang, D., Lin, Y., Zhou, Q., Lin, Y., Li, P., Niessner, R., Knopp, D. "Low-cost and highly sensitive immunosensing platform for aflatoxins using one-step competitive displacement reaction mode and portable glucometer-based detection." Analytical Chemistry 86 (2014) 11451-11458.

Wang, X., Niessner, R., Knopp, D. "Magnetic bead-based colorimetric immunoassay for Aflatoxin B1 using gold nanoparticles." Sensors 14 (2014) 21535-21548.

Oswald, S., Niessner, R., Knopp, D. "First experimental evidence for an enzyme-generated chemiluminescence-induced trans-cis isomerization of chip-immobilized zearalenone in a microfluidic cell of a biosensor" Luminescence 29 (2014) 23-24.

Sharma, A. K., Saini, M., Singh, S. D., Prakash, V., Das, A., Dasan, R. B., Pandey, S., Bohara, D., Galligan, T. H., Green, R. E., Knopp, D., Cuthbert, R. J. "Diclofenac is toxic to the steppe eagle Aquila nipalensis: widening the diversity of raptors threatened by NSAID misuse in South Asia" Bird Conserv. Int. (2014) 282-286.

Pschenitza, M., Hackenberg, R., Niessner, R., Knopp, D. "Analysis of benzo[a]pyrene in vegetable oil using molecularly imprinted solid phase extraction (MISPE) coupled with enzyme-linked immunosorbent assay (ELISA)" Sensors 14 (2014) 9720-9737.

Pschenitza, M., Gavrilova, E. S., Tarasov, S. A., Knopp, D., Niessner, R., Epstein, O. I. "Application of heterogeneous immunoassay for the quality control testing of release-active forms of diclofenac" Int. Immunopharmacol. 21 (2014) 225-230.

Eisold, U., Kupstat, A., Klier, D., Primus, P.-A., Pschenitza, M., Niessner, R., Knopp, D., Kumke, M. U. "Probing the physicochemical interactions of 3-hydroxy-benzo[a]pyrene with different monoclonal and recombinant antibodies by use of fluorescence line-narrowing spectroscopy" Anal. Bioanal. Chem. 406 (2014) 3387-3394.

Speranskaya, E. S., Beloglazova, N. V., Lenain, P., De Saeger, S., Wang, Z., Zhang, S., Hens, Z., Knopp, D., Niessner, R., Potapkin, D. V., Goryacheva, I. Yu. "Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay" Biosens. Bioelectron. 53 (2014) 225-231.

Tang, D., Liu, B., Niessner, R., Li, P., Knopp, D. "Target-induced displacement reaction accompanying cargo release from magnetic mesoporous silica nanocontainers for fluorescence immunoassay" Anal. Chem. 85 (2013), 10589-10596.

Beloglazova, N. V., Shmelin, P. S., Speranskaya, E. S., Lucas, B., Helmbrecht, C., Knopp, D., Niessner, R., De Saeger, S., Goryacheva, I. Yu. "Quantum dot loaded liposomes as new luminescent labels for immunoassay" Anal. Chem. 85 (2013) 7197-7204.

Oswald, S., Karsunke, X.Y.Z., Dietrich, R., Märtlbauer, E., Niessner, R., Knopp, D. "Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals" Anal. Bioanal. Chem. 405 (2013) 6405-6415.

Li, D., Ying, Y., Wu, J., Niessner, R., Knopp, D. "Comparison of monomeric and polymeric horseradish peroxidase as labels in competitive ELISA for small molecule detection" Microchimica Acta 180 (2013) 711-717.

Tang, D., Hou, L., Niessner, R., Xu, M., Gao, Z., Knopp, D. "Multiplexed electrochemical immunoassay of biomarkers using metal sulfide quantum dot nanolabels and trifunctionalized magnetic beads" Biosens. Bioelectron. 46 (2013) 37-43.

Zhang, B., Tang, D., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Anodic-stripping voltametric immunoassay for ultrasensitive detection of low-abundance proteins using quantum dot aggregated hollow microspheres" Chem. Eur. J. 19 (2013) 2496-2503.

Yu, H.-W., Kim, I. S., Niessner, R., Knopp, D. "Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels" Analytica Chimica Acta 750 (2012) 191-198.

Zhang, B., Liu, B., Tang, D., Niessner, R., Chen, G., Knopp, D. "DNA-based hybridization chain reaction for amplified bioelectronic signal and ultrasensitive detection of proteins" Anal. Chem. 84 (2012) 5392-5399.

Wang, Y., Yang, H., Pschenitza, M., Niessner, R., Li, Y., Knopp, D., Deng, A. "Highly sensitive and specific determination of mercury(II) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody" Anal. Bioanal. Chem. 403 (2012) 2519-2528.

Tang, J., Tang, D., Niessner, R., Knopp, R., Chen, G. "Hierarchical dendritic gold microstructure-based aptasensor for ultrasensitive electrochemical detection of thrombin using functionalized mesoporous silica nanospheres as signal tags" Analytica Chimica Acta 720 (2012) 1-8.

Karsunke, X.Y.Z., Wang, H., Weber, E., McLean, M.D., Niessner, R., Hall, J.C., Knopp, D. "Development of single-chain variable fragment (scFv) antibodies against hapten benzo[a]pyrene: a binding study" Anal. Bioanal. Chem. 402 (2012) 499-507.

Saini, M, Taggert, M. A., Knopp, D., Upreti, S., Swarup, D., Das, A., Gupta, P. K., Niessner, R., Prakash, V., Mateo, R., Cuthbert, R. J. "Detecting diclofenac in livestock carcasses in India with an ELISA: A tool to prevent widespread vulture poisoning" Environ. Pollut. 160 (2012) 11-16.

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