Prof. Dietmar Knopp

Head of group



Education (degrees, date, universities):

University of Leipzig (1972-1976)

Diploma in Biochemistry (1976), University of Leipzig
Title (translated): Investigations on the interaction of yeast alcohol dehydrogenase and its hydrophobic substrates
(Prof. H. Aurich)

Doctorate in Biochemistry (1985), University of Leipzig
Title (translated): Development and application of a radioimmunoassay for 2,4-dichlorophenoxyacetic acid
(Prof. P. Nuhn)

University lecturing qualification (habilitation) in Applied Biochemistry (1992), University Halle-Wittenberg
Title (translated): The immunological determination of 2,4-D in body fluids of exposed persons - a biological exposure test for the calculation of individual health risk
(Prof. P. Nuhn)

Postgraduation (1982-1986) Academy of physicians education, Berlin
Topic (translated): Medical toxicology/Toxicological chemistry

Visiting Scientist (1989), Laboratory of Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands
Topic: Immunoaffinity chromatography
(Dr. R. Stephany)


Research scientist at the Department of Environmental Toxicology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1976-1989)

Head of the Department of Experimental Toxicology and Epidemiology, Research Institute of Hygiene and Microbiology, Bad Elster, Germany (1989-1990)

Since 1991: Research Group at the Technical University of Munich, Institute of Hydrochemistry and Chemical Balneology (Prof. Dr. R. Niessner)

2002: Position of Academic Director

2005: Appointment apl. Professor


Membership of Editorial Boards:


  - Ecotoxicology and Environmental Safety

  - Chromatographia

  - International Journal of Environmental Research and Public Health

  - Guest Editor for Special Issue "Biochips" of the journal Sensors (ISSN 1424-8220) (2012)


Complete List for download

Period 2009 - 2013

Speranskaya, E. S., Beloglazova, N. V., Lenain, P., De Saeger, S., Wang, Z., Zhang, S., Hens, Z., Knopp, D., Niessner, R., Potapkin, D. V., Goryacheva, I. Yu. "Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay" Biosensors & Bioelectronics 53 (2014) 225-231.

Tang, D., Liu, B., Niessner, R., Li, P., Knopp, D. "Target-induced displacement reaction accompanying cargo release from magnetic mesoporous silica nanocontainers for fluorescence immunoassay" Anal. Chem. 85 (2013), 10589-10596.

Beloglazova, N. V., Shmelin, P. S., Speranskaya, E. S., Lucas, B., Helmbrecht, C., Knopp, D., Niessner, R., De Saeger, S., Goryacheva, I. Yu. "Quantum dot loaded liposomes as new luminescent labels for immunoassay" Anal. Chem. 85 (2013) 7197-7204.

Oswald, S., Karsunke, X.Y.Z., Dietrich, R., Märtlbauer, E., Niessner, R., Knopp, D. "Automated regenerable microarray-based immunoassay for rapid parallel quantification of mycotoxins in cereals" Anal. Bioanal. Chem. 405 (2013) 6405-6415.

Li, D., Ying, Y., Wu, J., Niessner, R., Knopp, D. "Comparison of monomeric and polymeric horseradish peroxidase as labels in competitive ELISA for small molecule detection" Microchimica Acta 180 (2013) 711-717.

Tang, D., Hou, L., Niessner, R., Xu, M., Gao, Z., Knopp, D. "Multiplexed electrochemical immunoassay of biomarkers using metal sulfide quantum dot nanolabels and trifunctionalized magnetic beads" Biosens. Bioelectron. 46 (2013) 37-43.

Zhang, B., Tang, D., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Anodic-stripping voltametric immunoassay for ultrasensitive detection of low-abundance proteins using quantum dot aggregated hollow microspheres" Chem. Eur. J. 19 (2013) 2496-2503.

Yu, H.-W., Kim, I. S., Niessner, R., Knopp, D. "Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels" Analytica Chimica Acta 750 (2012) 191-198.

Zhang, B., Liu, B., Tang, D., Niessner, R., Chen, G., Knopp, D. "DNA-based hybridization chain reaction for amplified bioelectronic signal and ultrasensitive detection of proteins" Anal. Chem. 84 (2012) 5392-5399.

Wang, Y., Yang, H., Pschenitza, M., Niessner, R., Li, Y., Knopp, D., Deng, A. "Highly sensitive and specific determination of mercury(II) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody" Anal. Bioanal. Chem. 403 (2012) 2519-2528.

Tang, J., Tang, D., Niessner, R., Knopp, R., Chen, G. "Hierarchical dendritic gold microstructure-based aptasensor for ultrasensitive electrochemical detection of thrombin using functionalized mesoporous silica nanospheres as signal tags" Analytica Chimica Acta 720 (2012) 1-8.

Karsunke, X.Y.Z., Wang, H., Weber, E., McLean, M.D., Niessner, R., Hall, J.C., Knopp, D. "Development of single-chain variable fragment (scFv) antibodies against hapten benzo[a]pyrene: a binding study" Anal. Bioanal. Chem. 402 (2012) 499-507.

Saini, M, Taggert, M. A., Knopp, D., Upreti, S., Swarup, D., Das, A., Gupta, P. K., Niessner, R., Prakash, V., Mateo, R., Cuthbert, R. J. "Detecting diclofenac in livestock carcasses in India with an ELISA: A tool to prevent widespread vulture poisoning" Environ. Pollut. 160 (2012) 11-16.

Beloglazova, N. V., Goryacheva, I. Yu., Niessner, R., Knopp, D. "Comparison of different labels for non-instrumental gel-based immunoassay" Microchimica Acta 175 (2011) 361-367.

Karsunke, X. Y. Z., Pschenitza, M., Rieger, M., Weber, E., Niessner, R., Knopp, D. "Screening and characterization of new monoclonal anti-benzo[a]pyrene antibodies using automated flow-through microarray technology" J. Immunol. Methods 371 (2011) 81-90.

Beloglazova, N. V., Goryacheva, I. Y., de Saeger, S., Scippo, M. L., Niessner, R., Knopp, D. "New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical test column test" Talanta 85 (2011) 151-156.

Tang, J., Tang, D., Niessner, R., Chen, G., Knopp, D. "Magneto-controlled graphene immunosensing platform for simultaneous multiplexed electrochemical immunoassay using distinguishable signal tags" Anal. Chem. (2011) 5407-5414.

Tang, J., Tang, D., Niessner, R., Knopp, D. "A novel strategy for ultra-sensitive electrochemical immunoassay of biomarkers by coupling multifunctional iridium oxide (IrOx) nanospheres with catalytic recycling of self-produced reactants" Anal. Bioanal. Chem. (2011) 2041-2051.

Sauceda-Friebe, J. C., Karsunke, X. Y. Z., Vazac, S., Biselli, S., Niessner, R., Knopp, D. "Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection" Analytica Chimica Acta 689 (2011) 234-242.

Tang, D.,Yu, Y., Niessner, R., Miro, M., Knopp, D. "Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles" Analyst 135 (2010) 2661-2667. 

Maiolini, E., Knopp, D., Niessner, R., Eremin, S., Bolelli, L., Ferri, E. N., Girotti, S. "Chemiluminescence ELISA for BTEX determination in water and soil" Analytical Sciences 26 (2010) 773-777.

Lin, Z., Sauceda-Friebe, J. C., Lin, J.-M., Niessner, R., Knopp, D. "Double-codified nanogold particles based automated flow-through chemiluminescence immunoassay for 2,4-dinitrotoluene" Analytical Methods 2 (2010) 824-830.

Kupstat, A., Knopp, D., Niessner, R., Kumke M.U. "Novel intramolecular energy transfer probe for the detection of Benzo[a]pyrene metabolites in a homogeneous competitive fluorescence immunoassay" J. Phys. Chem. B 114 (2010) 1666-1673.

Tang, D., Sauceda, J.C., Lin, Z., Ott, S., Basova, E., Goryacheva, I., Biselli, S., Lin, J., Niessner, R., Knopp, D. "Magnetic nanogold microsperes-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food" Biosensors & Bioelectronics 25 (2009) 514-518. 

Tang, D., Zhong, Z., Niessner, R., Knopp, D. "Double-codified gold nanolabels with enhanced electrochemical immunoassay for rapid detection of aflatoxin B1 in food using multifunctional magnetic beads as probes" The Analyst 134 (2009) 1509-1728.

Knopp, D., Tang, D., Niessner, R. "Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles" Analytica Chimica Acta 647 (2009) 14-30.

Rieger, M., Cervino, C., Sauceda, J. C., Niessner, R., Knopp, D. "Efficient Hybridoma Screening Technique Using Capture Antibody Based Microarrays" Anal. Chem. 81 (2009) 2373-2377.

Tang, D., Niessner, R., Knopp, D. "Flow-injection electrochemical immunosensor for the detection of human IgG based on glucose oxidase-derived biomimetic interface" Biosensors and Bioelectronics 24 (2009) 2125 - 2130.

(c) 2014 Institute of Hydrochemistry, Technische Universität München, Marchioninistr. 17, D-81377 München
phone: +49 89 2180-78231, fax: +49 89 2180-78255, reinhard.niessner(at), Impressum